KUIMSe003-A-1

General

Cell Line

hPSCreg name KUIMSe003-A-1
Cite as:
KUIMSe003-A-1 (RRID:CVCL_T922)
Cell line type Human embryonic stem cell (hESC)
Similar lines
KUIMSe003-A
(KhES-3)
Last update 3rd September 2018
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_T922
Wikidata Q54899878

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Also have a look at the ethics information for the parental line KUIMSe003-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line KUIMSe003-A.

Culture Conditions

Feeder cells mouse embryo fibroblast cells
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
2-mercaptoetanol
FGF2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TDGF1
Yes
TRA 1-81
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX,+16

Other Genotyping (Cell Line)

Genetic Modification