KUIMSe003-A-1

General#

Cell Line

hPSCreg Name KUIMSe003-A-1
Cell line type Human embryonic stem cell (hESC)
Last update 3rd September 2018
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_T922

General Information

Publications
Is the cell line available in principle?
Yes
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells mouse embryo fibroblast cells
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
2-mercaptoetanol
FGF2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TDGF1
Yes
TRA 1-81
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX,+16

Other Genotyping (Cell Line)

Genetic Modification#