Generation of an integration-free iPSC line, ICCSICi005-A, derived from a Parkinson's disease patient carrying the L444P mutation in the GBA1 gene


The L444P mutation in the GBA1 gene which encodes β-glucocerebrosidase-1, is a major risk factor for developing Parkinson's disease (PD) and dementia with Lewy bodies (DLB). We report the generation and characterization of an induced pluripotent stem cell (iPSC) line derived from a female PD patient carrying the L444P/wt mutation. The iPSC line presented a normal morphology, expressed endogenous pluripotency markers, could be differentiated into endodermal, mesodermal and ectodermal cells, was free from Sendai vectors and reprogramming factors, had a normal karyotype and maintained the original GBA1 genotype. Thus, this iPSC line can serve to establish cellular models of PD. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Rodríguez-Traver E, Rodríguez C, Díaz-Guerra E, Arenas F, Araúzo-Bravo M, Orera M, Kulisevsky J, Moratalla R, Vicario C
Journal Stem cell research
Publication Date 2019 Oct;40:101578
PubMed 31539859
DOI 10.1016/j.scr.2019.101578

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