AICS-0005-050

General#

Cell Line

hPSCreg Name UCSFi001-A-1
Alternative name(s)
AICS-0005-050
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 20th November 2019
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Provider

Generator Allen Institute for Cell Science (AICS)
Owner Allen Institute for Cell Science (AICS)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA5843919
Allen Cell Collection AICS-0005 cl.50

General Information

Notes https://www.allencell.org/cell-catalog.html
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? No
Is clinical information available? Limited clinical information - EKG

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5843920

Ethics#

Also have a look at the ethics information for the parental line UCSFi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line UCSFi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line UCSFi001-A

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&hubClear=https://s3-us-west-2.amazonaws.com/downloads.allencell.org/genome-sequence/ucsc_hubs/AICS5/hub.txt
This cell line has undergone whole exome sequencing. Access exome variant track via the UCSC genome browser

Genetic Modification#

Genetic modifications not related to a disease
PXN (target)
Gene knock-in
12q24.23
EGFP
12q24.23
CRISPR-Cas9 methodology was used to introduce EGFP at the C-terminus of PXN
CRISPR-associated (CRISPR/Cas) System