WIBR3 Oct4-2A-GFP

General

Cell Line

hPSCreg name WIBRe001-A-2
Cite as:
WIBRe001-A-2 (RRID:CVCL_IU05)
Alternative name(s)
WIBR3 Oct4-2A-GFP
Cell line type Human embryonic stem cell (hESC)
Similar lines
WIBRe001-A-1
(WIBR3 Oct4-deltaPE-GFP)
WIBRe001-A
(WIBR38, WIBR3)
Last update 22nd May 2019
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Provider

Generator Whitehead Institute for Biomedical Research (WIBR)
Owner Whitehead Institute for Biomedical Research (WIBR)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA103887531
Cellosaurus CVCL_IU05
Wikidata Q54994043

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104129930

Ethics

Also have a look at the ethics information for the parental line WIBRe001-A .
Alternatives to consent

hESC Derivation

The source cell information can be found in the parental cell line WIBRe001-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzymatically
TrypLE
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
POU5F1 (OCT-4) (target)
Transgene expression
Nature Biotech paper describes the construction of this subclone. Briefly, hESC line WIBR#3 was engineered to contain eGFP preceded by a 2A sequence, fused in-frame following the last OCT4 codon in exon 5 of OCT4 (POU5F1).
TALEN
WIBR3_Oct4-2A-GFP_paper.pdf
Nature Biotech paper describes the construction of this subclone. Briefly, hESC line WIBR#3 was engineered to contain eGFP preceded by a 2A sequence, fused in-frame following the last OCT4 codon in exon 5 of OCT4 (POU5F1).