WIBR3 Oct4-2A-GFP

General#

Cell Line

hPSCreg Name WIBRe001-A-2
Alternative name(s)
WIBR3 Oct4-2A-GFP
Cell line type Human embryonic stem cell (hESC)
Last update 16th January 2018
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Provider

Generator Whitehead Institute for Biomedical Research (WIBR)
Owner Whitehead Institute for Biomedical Research (WIBR)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA103887531
Cellosaurus CVCL_IU05

General Information

Publications
Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104129930

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2008-11-17
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzymatically
TrypLE
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Unkown
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
POU5F1 (target)
Transgene expression
Nature Biotech paper describes the construction of this subclone. Briefly, hESC line WIBR#3 was engineered to contain eGFP preceded by a 2A sequence, fused in-frame following the last OCT4 codon in exon 5 of OCT4 (POU5F1).
TALEN
WIBR3_Oct4-2A-GFP_paper.pdf
Nature Biotech paper describes the construction of this subclone. Briefly, hESC line WIBR#3 was engineered to contain eGFP preceded by a 2A sequence, fused in-frame following the last OCT4 codon in exon 5 of OCT4 (POU5F1).