WIBR3 Oct4-deltaPE-GFP

General#

Cell Line

hPSCreg Name WIBRe001-A-1
Alternative name(s)
WIBR3 Oct4-deltaPE-GFP
Cell line type Human embryonic stem cell (hESC)
Last update 16th January 2018
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Provider

Generator Whitehead Institute for Biomedical Research (WIBR)
Owner Whitehead Institute for Biomedical Research (WIBR)
Derivation country United States

External Databases

BioSamples SAMEA103887532
Cellosaurus CVCL_IU04

General Information

Publications
Projects
Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104129930

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2008-11-17
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzymatically
TrypLE
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Unkown
Self-renewal
Negative
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unkown

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
POU5F1 (target)
Transgene expression
Original paper describes the OCT4-GFP reporter construct containing a 1 kb deletion of the proximal enhancer. The reporter construct is a 2A-GFP sequence in frame with the 3'UTR of OCT4.
TALEN
WIBR3-deltaPE_Oct4_GFP§WIN1-paper_.pdf
Original paper describing the OCT4-GFP reporter construct containing a 1 kb deletion of the proximal enhancer. The reporter construct is a 2A-GFP sequence in frame with the 3'UTR of OCT4.