WIBR3 Oct4-deltaPE-GFP
WIBRe001-A-1
General
Cell Line |
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| hPSCreg name | WIBRe001-A-1 |
| Cite as: | WIBRe001-A-1 (RRID:CVCL_IU04) |
| Alternative name(s) |
WIBR3 Oct4-deltaPE-GFP
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WIBRe001-A-2 (WIBR3 Oct4-2A-GFP) WIBRe001-A (WIBR38, WIBR3) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator | Whitehead Institute for Biomedical Research (WIBR) |
| Owner | Whitehead Institute for Biomedical Research (WIBR) |
| Derivation country | United States |
External Databases |
|
| BioSamples | SAMEA103887532 |
| Cellosaurus | CVCL_IU04 |
| Wikidata | Q54994044 |
General Information |
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| Publications |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | unknown |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | no |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA104129930 |
Ethics
Also have a look at the ethics information for the parental line
WIBRe001-A
.
| Alternatives to consent |
hESC Derivation
|
The source cell information can be found in the parental cell line
WIBRe001-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Self-renewal
Negative
Endoderm
Negative
Mesoderm
Negative
Ectoderm score
Negative
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Unknown
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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