Generation of an induced pluripotent stem cell line (ZJULLi003-A) from a hypertrophic cardiomyopathy patient carrying MYH7/c.4384G > A mutation


Hypertrophic cardiomyopathy (HCM) is an autosomal dominant inherited cardiovascular disease characterized by left ventricular hypertrophy and cardiomyocyte disarray. In this study, a skin biopsy was obtained from a HCM patient, who carried a missense mutation (c.4384G > A; p.E1462K) in the myosin heavy chain 7 (MYH7) gene. The skin fibroblasts were subsequently reprogrammed with a non-integrated Sendai viral method to generate a patient-specific induced pluripotent stem cell (iPSC) line. The generated iPSC line showed typical morphology and normal karyotype, expressed pluripotency markers, and was capable to differentiate into three germ layers. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhou J, Sun Y, Wang H, Wang H, Guo F, Chen X, Gong T, Jiang C, Liang P
Journal Stem cell research
Publication Date 2022 Oct;64:102883
PubMed 35944310
DOI 10.1016/j.scr.2022.102883

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