Generation of two hiPSC lines, (DMBi003-A and DMBi004-A), by reprogramming peripheral blood mononuclear cells and fibroblast-like synoviocytes from rheumatoid arthritis patients

Summary

Rheumatoid arthritis (RA) is a chronic inflammatory disease that primarily affects joints but should be considered as a syndrome that also includes extra-articular manifestations and comorbidities. Human-derived induced pluripotent stem cells (hiPSCs) and their differentiated derivatives may be of special interest in the investigation of complex pathophysiology of RA. In this study, we demonstrate and compare the generation of hiPSC from peripheral blood mononuclear cells (PBMC) and fibroblast-like synoviocytes (FLS) from RA patients. Application of three-dimensional cardiac microtissues constructed from RA specific iPSC-derivatives may be a useful approach to investigate RA comorbidities and cardiac protection or toxicity of anti-rheumatic drugs. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Wolnik J, Kubiak G, Skoczyńska M, Wiland P, Fearon U, Veale D, Dulak J, Biniecka M
Journal Stem cell research
Publication Date 2022 Aug 3;64:102886
PubMed 35973333
DOI 10.1016/j.scr.2022.102886

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