UKAi001-C-1

hsc3_hiPS_40_16_6, IRF8-/- iPS3

General#

Cell Line

hPSCreg Name
UKAi001-C-1
Alternative name(s)
hsc3_hiPS_40_16_6, IRF8-/- iPS3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th February 2021
Notes Sontag et al., Stem Cells 35, 898-908, 2017. PMID:28090699
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Provider

Generator Universitätsklinikum Aachen (UKA)
Owner Universitätsklinikum Aachen (UKA)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA8192628
CLO CLO_0103263
Cellosaurus CVCL_A5GS
Wikidata Q107117210

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
systemic mastocytosis
mast cell leukemia (DOID:9254)
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • SMCD - systemic mast cell disease
  • systemic tissue mast cell disease
Genetic variants
KRAS (target)
12p12.1
KRAS G436A
Heterozygous
VCV000197243.1
PMID:28090699
KRAS G436A mutation (NM_033360.4) is a disease-associated mutation and not a disease-causing mutation. Samples were from a mastocytosis patient but the iPS cell clones did not contain the disease-causing mutation KIT D816V but rather a heterozygous KRAS G436A mutation, which we refer to as disease associated mutation.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA8133083

Ethics#

Also have a look at the ethics information for the parental line UKAi001-C .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Life Technologies

hIPSC Derivation#

General

The source cell information can be found in the parental cell line UKAi001-C.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Selection criteria for clones Human ES cell-like morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblasts
Passage method Enzymatically
Collagenase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knock out DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
human albumin
Yes
alpha fetoprotein
Yes
SOX 17
Yes
FOXA2
Yes
Hematopoietic Precursor Cell
Ont Id: CL_0008001
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
cardiac troponin T
Yes
PECAM1
Yes
CD34
Yes
CD43
Yes
CD45
Yes
ckit
Yes
MHC6
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
NES
Yes
beta III tubulin
Yes
PAX6
Yes
Sox1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
IRF8 (target)
Gene knock-out
IRF8 deletions were determined by Sanger Sequencing (Sontag et al., 2017, Supporting Information Figure 2A and B).
CRISPR-associated (CRISPR/Cas) System