Brain-derived neurotrophic factor increases cell number of neural progenitor cells derived from human induced pluripotent stem cells
Summary
The proliferative effect of BDNF was remarkably observed as an increase in Ki-67 positive cells. The cell number of hNPCs was significantly increased after BDNF treatment represented by cellular metabolic activity of the cells measured by MTT assay. This noticeable effect was statistically shown at 20 ng/ml of BDNF treatment. BDNF, however, did not promote neurite outgrowth but increased neuronal cell number. It was found that AG490 suppressed hNPCs proliferation. However, this inhibitor partially decreased BDNF-induced hNPCs proliferation. These results demonstrated the potential role of BDNF for the amelioration of AD through the increase of AD-derived hNPCs number. ©2021 Pansri et al.
| Authors | Pansri P, Phanthong P, Suthprasertporn N, Kitiyanant Y, Tubsuwan A, Dinnyes A, Kobolak J, Kitiyanant N |
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| Journal | PeerJ |
| Publication Date | 2021;9:e11388 |
| PubMed | 34026357 |
| PubMed Central | PMC8123227 |
| DOI | 10.7717/peerj.11388 |