Establishment of a CRISPR/Cas9-mediated ANP32A homozygous knockout human embryonic stem cell line
ANP32A is a member of acidic leucine-rich nuclear phosphoprotein 32 family, which is involved in diverse biochemical processes, including chromatin modification and remodeling. Here, we established the CRISPR/Cas9-mediated ANP32A homozygous knockout human embryonic stem cell (ESC) line to investigate the roles of ANP32A in pluripotency maintenance and differentiation process of human ESCs. This cell line shows the normal karyotype and typical stem cell morphology, in accordance with high expression of pluripotent genes and the differentiation potential in vitro. Consequently, the ANP32A knockout cell line provides a promising approach for investigating the roles of ANP32A in human ESC cell fate decisions. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
|Authors||Zhang C, Wei Y, Zhang Y, Zhang J, Xing Q, Zhou M, Shan Y|
|Journal||Stem cell research|
|Publication Date||2021 Apr;52:102234|