RC14, RC-14
RCe018-A
General#
Cell Line |
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| hPSCreg Name | RCe018-A |
| Alternative name(s) |
RC14, RC-14
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 10th April 2017 |
| User feedback | |
Provider |
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| Generator | Roslin Cells (RC) |
| Owner | Derivation of Clinical Grade hES Cells |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
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| Cellosaurus | CVCL_L203 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Ethics#
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Scotland A Research Ethics Committee |
| Approval number | 07/MRE00/56 |
hESC Derivation#
| Date of derivation | 2010-09-10 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Expansion status |
4
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| ICM morphology |
A
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| Trophectoderm morphology |
g
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| ZP removal technique | Spontaneous Hatching |
| Cell isolation | Trophoectoderm and ICM isolated |
| Cell seeding | Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination |
| Derived under xeno-free conditions? |
Yes |
| Derivation under GMP? |
Yes |
| Available as clinical grade? |
Yes |
Culture Conditions#
| Surface coating | Matrigel/Geltrex | |||||||||
| Feeder cells |
dermal fibroblast Cellfinder Ont Id: CL_0002620 |
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| O2 Concentration | 5 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
StemPro® hESC SFM
Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-1 |
No |
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Morphology pictures
Morphology of colonies of passages 9 and 27
Differentiation Potency
Microbiology / Virus Screening |
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Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
47XY,+8
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Other Genotyping (Cell Line) |
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