Establishment of a human iPSC line XMDYYYi001-A from a patient with Becker muscular dystrophy harboring duplications of exons 2-19 in dystrophin gene
Summary
Becker muscular dystrophy (BMD) is an X-linked recessive muscular disorder caused by mutations in the dystrophin. We generated a human iPSC line from peripheral blood mononuclear cells (PBMCs) of a patient with duplications of exons 2-19 in the dystrophin. The PBMCs were reprogrammed using the episomal reprogramming plasmids contained a combination of expressions of human OCT4, SOX2, NANOG, LIN28, C-MYC, KLF4 and SV40LT. We conducted the tests on the iPSCs including Karyotype analysis, expressed pluripotency markers and teratoma forming three germ layers. The iPSC line is a useful cell model to further research on genetic treatment or new therapeutic drugs. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wang D, Lin J, Jin M, Wang N |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102298 |
| PubMed | 33799273 |
| DOI | 10.1016/j.scr.2021.102298 |