Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system


Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Luo Q, Wei C, Long Y, Zhang M, Shan W, Li H, Cai S, Xu Y, Qian P, Huang H
Journal Stem cell research
Publication Date 2021 May;53:102350
PubMed 34087984
DOI 10.1016/j.scr.2021.102350

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