HuES3
HVRDe003-A
General
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | Please see the NIH Stem Cell Registry https://grants.nih.gov/stem_cells/registry/current.htm?id=34 HUES 3 was given NIH Approval Number NIHhESC-09-0016 |
| Alternative consent approval number | NIHhESC-09-0016 |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| How may genetic information associated with the cell line be accessed? | |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Harvard University Committee on the Use of Human Subjects |
| Approval number |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| PGD Embryo? |
No |
| Expansion status |
4
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| ICM morphology |
A
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| Trophectoderm morphology |
g
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| ZP removal technique | Chemical |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
Mouse Embryonic Fibroblast Cell Cellfinder Ont Id: EFO_0004040 |
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| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 10 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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| TDGF1 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 26
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Other Genotyping (Cell Line) |
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