Valproic acid promotes the in vitro differentiation of human pluripotent stem cells into spermatogonial stem cell-like cells
Summary
VPA robustly promoted the differentiation of human pluripotent stem cells into SSCLCs, which involved the upregulated genes in Wnt signaling pathway and epigenetic changes. hiPSCs from NOA patients showed decreased SSCLC induction efficiency and Wnt signaling pathway gene expression, suggesting that SSC depletion in azoospermia testes might be associated with inactivation of Wnt signaling pathway. Our developed SSCLC induction protocol provides a reliable tool and model to study human germ cell development and male infertility. © 2021. The Author(s).
| Authors | Wang X, Qu M, Li Z, Long Y, Hong K, Li H |
|---|---|
| Journal | Stem cell research & therapy |
| Publication Date | 2021 Oct 29;12(1):553 |
| PubMed | 34715904 |
| PubMed Central | PMC8555208 |
| DOI | 10.1186/s13287-021-02621-1 |