HS368
KIe015-A
General#
Cell Line |
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| hPSCreg Name | KIe015-A |
| Alternative name(s) |
HS368
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 11th April 2019 |
| User feedback | |
Provider |
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| Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
| Derivation country | Sweden |
External Databases |
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| Cellosaurus | CVCL_C208 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclones | |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Regional ethical committee |
hESC Derivation#
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Chemical |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions#
| Feeder cells |
Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| TDGF1 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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