HS368
KIe015-A
General#
Cell Line |
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hPSCreg Name | KIe015-A |
Alternative name(s) |
HS368
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 28th July 2017 |
User feedback | |
Provider |
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Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
Derivation country | Sweden |
External Databases |
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Cellosaurus | CVCL_C208 |
General Information |
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Publications |
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Is the cell line available in principle? |
Yes |
Subclones |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases |
Ethics#
We are currently revising this section.hESC Derivation#
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
No |
ZP removal technique | Chemical |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Culture Conditions#
Feeder cells |
Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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TDGF1 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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