An integration-free iPSC line, ICCSICi007-A, derived from a female Alzheimer's disease patient with the APOE-ε4/ε4 alleles


The epsilon4 (ε4) allele of the APOE gene, which encodes the apolipoprotein E4 (ApoE4), is the strongest genetic risk factor known for late-onset Alzheimer´s disease (LOAD). Here, we present the characterization of an iPSC line generated from dermal fibroblasts of a female AD patient using Sendai viral vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The iPSCs maintained the original genotype, a normal karyotype, were free from Sendai viral vectors and reprogramming factors, presented a normal morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency. Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Díaz-Guerra E, Rodríguez-Traver E, Moreno-Jiménez EP, de Rojas I, Rodríguez C, Orera M, Hernández I, Ruiz A, Vicario C
Journal Stem cell research
Publication Date 2019 Dec;41:101588
PubMed 31698192
DOI 10.1016/j.scr.2019.101588

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