AG09393A clone 3, AG09393A-3
CBRCULi014-A
General
Cell Line |
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| hPSCreg name | CBRCULi014-A |
| Cite as: | CBRCULi014-A (RRID:CVCL_C9J2) |
| Alternative name(s) |
AG09393A clone 3, AG09393A-3
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 23rd January 2024 |
| User feedback | |
Provider |
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| Generator | CERVO Brain Research Centre-Université Laval (CBRCUL) |
External Databases |
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| Cellosaurus | CVCL_C9J2 |
| BioSamples | SAMEA114857239 |
| Wikidata | Q123030872 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | White |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46 XX normal
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External Databases (Donor) |
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| BioSamples | SAMEA115116814 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | https://coriell.org/ |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent pertain to a specific research project? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | |
| Approval number | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | CHUQ Ethical Committee |
| Approval number | 2018-4172 |
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation
General |
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| Source cell line name | AG09393 |
| Source cell type |
Human cell line from tissue infected with Epstein-Barr virus, resembling a lymphoblast.
Synonyms
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| Source cell line vendor | Coriell Institute for Medical Research |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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| Notes on reprogramming vector detection | Vector detection was carried on cells at P19 |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| Medium |
mTeSR™ Plus
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| DNMT3B |
Yes |
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| TERT |
Yes |
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| CRIPTO/TDGF1 |
Yes |
Morphology pictures
CBRCULi014-A morphology
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
XX Loss of 414 kbp at chromosome 2 q22.1
Passage number: 4
Karyotyping method:
Molecular karyotyping by SNP array
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Other Genotyping (Cell Line) |
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