UOCi003-A-1

A18 GPiPSC 31-1

General

Cell Line

hPSCreg name UOCi003-A-1
Cite as:
UOCi003-A-1 (RRID:CVCL_D0P5)
Alternative name(s)
A18 GPiPSC 31-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UOCi003-A
(GPiPSC 31-1)
Donor's gene variants:
CELF2
Donor diseases:
Simplified gyral pattern
Last update 17th August 2023
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Provider

Generator University of Calgary (UOC)
Owner University of Calgary (UOC)

External Databases

BioSamples SAMEA114285740
Cellosaurus CVCL_D0P5
Wikidata Q123033754

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Cortical gyral simplification
Genetic variants
CELF2 (target)
chr10:g.11370967 G>A
NM_001025077.3: c.1554G>A, p.(Arg493His)
Heterozygous

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA114285737

Ethics

Also have a look at the ethics information for the parental line UOCi003-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line UOCi003-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
CD184
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
NCAM1
Yes
Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Pax6
Yes
Nestin
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Genetic modifications
CELF2 (target)
Isogenic modification
chr10:g.11370967 A>G
NM_001025077.3: c.1554A>G, p.(Hia493Arg)
Heterozygous
Repaired