BGU02iGRIN2D CTR
BGUi012-A
General#
Cell Line |
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| hPSCreg Name | BGUi012-A |
| Alternative name(s) |
BGU02iGRIN2D CTR
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 4th February 2021 |
| User feedback | |
Provider |
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| Generator | Ben Gurion University of the Negev (BGU) |
| Owner | Ben Gurion University of the Negev (BGU) |
| Distributors | |
| Derivation country | Israel |
External Databases |
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| BioSamples | SAMEA8073005 |
| CLO | CLO_0103182 |
| Cellosaurus | CVCL_A4YL |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
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Donor Information#
General Donor Information |
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| Sex | male |
| Ethnicity | Jewish Ashkenazi |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| All cell lines of this donor's relatives |
Has son:
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External Databases (Donor) |
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| BioSamples | SAMEA8152186 |
Ethics#
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | Dr. Moran Hausman-Kedem, Sackler Faculty of Medicine, Tel Aviv University |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | Dr. Moran Hausman-Kedem, Sackler Faculty of Medicine, Tel Aviv University |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| a non-profit company? | Yes |
| a for-profit corporation? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation#
General |
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| Source cell type |
Fibroblasts |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
| Feeder cells |
No |
| Medium |
Other medium:
Base medium: NutriStem® hPSC XF Medium
Main protein source: Serum concentration: % |
Characterisation#
Analysis of Undifferentiated Cells
Morphology pictures
BGUi012-A morphology
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| AFP |
Yes |
Morphology
AFP Immunostaining
In vitro spontaneous differentiation
| Marker | Expressed |
| ACTA2 |
Yes |
Morphology
ACTA2 Immunostaining
In vitro spontaneous differentiation
| Marker | Expressed |
| NEFL |
Yes |
Morphology
NEFL Immunostaining
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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