CHOPJMML1854

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name CHOPi001-A
Alternative name(s)
CHOPJMML1854
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th November 2018
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Provider

Generator The Children's Hospital of Philadelphia (CHOP)

External Databases

Cellosaurus CVCL_VN17
BioSamples SAMEA5291610

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Juvenile Myelomonocytic Leukemia
Synonyms
  • JMML
  • Juvenile Myelomonocytic Leukemia
  • juvenile myelomonocytic leukemia
  • Juvenile myelomonocytic leukemia
  • JCML
  • Juvenile Chronic Myeloid Leukemia
  • Juvenile Chronic Myelogenous Leukemia
  • Chronic Myelomonocytic Leukemia
show more synonyms

External Databases (Donor)

BioSamples SAMEA5291611

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
mononuclear cell
A cell having only one nucleus, especially: MONOCYTE.
Source cell origin
Bone Marrow
The tissue occupying the spaces of bone. It consists of blood vessel sinuses and a network of hematopoietic cells which give rise to the red cells, white cells, and megakaryocytes.
Synonyms
  • BONE MARROW
  • Bone Marrow
  • Reticuloendothelial System, Bone Marrow
  • bone marrow
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 10 ng/ml
beta mercaptoethanol 0.1 mM

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
TRA 1-81
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
T antigen
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
T antigen
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
OTX2
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)