HS975

General#

Cell Line

hPSCreg Name KIe032-A
Alternative name(s)
HS975
Cell line type Human embryonic stem cell (hESC)
Last update 16th January 2018
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Provider

Generator Karolinska Institutet (KI)
Derivation country Sweden

External Databases

Cellosaurus CVCL_9S00

General Information

Publications
Projects
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2012-03-19
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 6
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Spontaneous Hatching
Cell isolation Mechanical
Cell seeding Isolated ICM
Derived under xeno-free conditions?
Yes
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Laminin
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Cell Line)