Royan H9

General#

Cell Line

hPSCreg Name RIe009-A
Alternative name(s)
Royan H9
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Royan Institute (RI)
Derivation country Iran

External Databases

Cellosaurus CVCL_9390

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
L-Glutamnine 2 mM
β-mercaptoethanol 0.1 mM
non-essential Amino Acid 1 %
Penicillin 100 Units/ml
Streptomycin 100 µg/ml
insulin-transferrin-selenite

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
45, XX, der(13;14)(q10;q10)

Other Genotyping (Cell Line)