AND-1
ESe029-A
General#
Cell Line |
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hPSCreg Name | ESe029-A |
Alternative name(s) |
AND-1
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 22nd May 2019 |
User feedback | |
Provider |
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Generator | Spanish Stem Cell Bank (ES) |
Owner | Andalusian Stem Cell Bank |
Distributors | |
Derivation country | Spain |
External Databases |
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BioSamples | SAMEA7772668 |
Cellosaurus | CVCL_B526 |
General Information |
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Publications | |
Projects | |
* Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: allowed
Commercial: allowed
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Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA7771918 |
Ethics#
Alternatives to consent are available? | Yes |
Alternatives to consent |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Health Department Junta de Andalucia -ISCIII |
Approval number | ATO 1205 |
hESC Derivation#
Date of derivation | 2007-04-23 |
Embryo stage | Morula |
Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
PGD Embryo? |
No |
Expansion status |
3![]() |
ICM morphology |
B![]() |
Trophectoderm morphology |
b![]() |
ZP removal technique | Enzymatic |
Cell isolation | Laser |
Cell seeding | Isolated ICM |
Derived under xeno-free conditions? |
No |
Derivation under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
bone marrow mesenchymal stem cells |
Passage method |
Enzymatically
TrypLE
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: KO DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Morphology pictures
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
14
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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