AND-1
ESe029-A
General#
Cell Line |
|
| hPSCreg Name | ESe029-A |
| Alternative name(s) |
AND-1
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 11th September 2017 |
| User feedback | |
Provider |
|
| Generator | Spanish Stem Cell Bank (ES) |
| Owner | Andalusian Stem Cell Bank |
| Distributors | |
| Derivation country | Spain |
External Databases |
|
| BioSamples | SAMEA7772668 |
| Cellosaurus | CVCL_B526 |
General Information |
|
| Publications | |
| Projects | |
| Is the cell line available in principle? | Available, without restrictions |
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA7771918 |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Health Department Junta de Andalucia -ISCIII |
| Approval number | ATO 1205 |
hESC Derivation#
| Date of derivation | 2007-04-23 |
| Embryo stage | Morula |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
B
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Enzymatic |
| Cell isolation | Laser |
| Cell seeding | Isolated ICM |
| Derived under xeno-free conditions? |
No |
| Derivation under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
bone marrow mesenchymal stem cells |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: KO DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
|
|
||
| NANOG |
Yes |
|
|
|||
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| SSEA-3 |
Yes |
|
|
|||
| SSEA-4 |
Yes |
|
|
|||
| TRA 1-60 |
Yes |
|
|
|||
| TRA 1-81 |
Yes |
|
|
|||
| ZFP42 (REX-1) |
Unkown |
|
|
|||
| DNMT3B |
Unkown |
|
||||
| GDF3 |
Unkown |
|
||||
| nodal |
Unkown |
|
||||
| podxl |
Unkown |
|
||||
| xist |
Unkown |
|
||||
| LEFTY1 |
Unkown |
|
||||
| foxd3 |
Unkown |
|
Morphology pictures
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
14
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.