AND-1

General#

Cell Line

hPSCreg Name ESe029-A
Alternative name(s)
AND-1
Cell line type Human embryonic stem cell (hESC)
Last update 11th September 2017
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Provider

Generator Spanish Stem Cell Bank (ES)
Owner Andalusian Stem Cell Bank
Distributors
Derivation country Spain

External Databases

BioSamples SAMEA7772668
Cellosaurus CVCL_B526

General Information

Publications
Projects
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA7771918

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2007-04-23
Embryo stage Morula
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation Laser
Cell seeding Isolated ICM
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells bone marrow mesenchymal stem cells
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: KO DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
ZFP42 (REX-1)
Unkown
DNMT3B
Unkown
GDF3
Unkown
nodal
Unkown
podxl
Unkown
xist
Unkown
LEFTY1
Unkown
foxd3
Unkown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesenchymal Cell
Ont Id: CL_0008019
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Blood Cell
Ont Id: CL_0000081
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 14

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)