AND-1
ESe029-A
General#
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
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| BioSamples | SAMEA7771918 |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Health Department Junta de Andalucia -ISCIII |
| Approval number | ATO 1205 |
hESC Derivation#
| Date of derivation | 2007-04-23 |
| Embryo stage | Morula |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
B
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Enzymatic |
| Cell isolation | Laser |
| Cell seeding | Isolated ICM |
| Derived under xeno-free conditions? |
No |
| Derivation under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
bone marrow mesenchymal stem cells |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: KO DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Morphology pictures
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
14
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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