General

Cell Line
hPSCreg name	SDUe005-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	SDUe005-A (RRID:CVCL_A279)
Alternative name(s)	KMEB5
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	22nd May 2019
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Provider
Generator	University of Southern Denmark (SDU) Contact: Medicinsk Bioteknologisk Center
Derivation country	Denmark
External Databases
Cellosaurus	CVCL_A279
Wikidata	Q54952734
General Information
Publications	Prokhorova TA et al. Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. Stem cells and development. 2009 Jan-Feb;18(1):47-54. Harkness L et al. Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):259-68.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

Date of derivation	2006-03-29
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	No
Expansion status	3 Stephenson 2007
ICM morphology	A Stephenson 2007
Trophectoderm morphology	a Stephenson 2007
ZP removal technique	Spontaneous Hatching
Cell isolation	None
Cell seeding	Embryo

Culture Conditions

Surface coating	Gelatin
Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
Passage method	Mechanically
CO2 Concentration	5 %
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements Supplement Amount Unit L-Glutamine

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
SOX2	Yes
SSEA-4	Yes
TRA 1-81	Yes
Alkaline Phosphatase	Yes
beta Tubulin-3	Yes
nodal	Yes
SSEA-1	No
TRA 1-60	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vivo teratoma

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vivo teratoma

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vivo teratoma

In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XY Passage number: 27
Other Genotyping (Cell Line)