PB09
UPSFRi008-A
General#
Cell Line |
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| hPSCreg Name | UPSFRi008-A |
| Alternative name(s) |
PB09
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th December 2017 |
| User feedback | |
Provider |
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| Generator |
Université Paris-Sud 11 (UPSFR)
Contact:
ESTeam Paris Sud |
| Derivation country | France |
External Databases |
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| Cellosaurus | CVCL_C353 |
General Information |
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| Publications |
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| Is the cell line available in principle? |
Yes |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics#
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: |
| Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? | Yes |
| Contact information / weblink |
| Alternatives to consent |
| Alternative consent approval number |
| Details on restriction to research project |
| Policy for access to genetic information |
| Please describe how access is provided: |
| Contact data, institution, or website: |
| Name of accrediting authority involved? | Local Institutional Review Board |
| Approval number |
| Name of accrediting authority involved? | Local Institutional Review Board |
| Approval number |
| Please describe: |
| Further constraints on use |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
| Constraints for use or distribution |
hIPSC Derivation#
General |
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| Source cell type |
amniocyteA cell of a fetus which is suspended in the amniotic fluid. Amniocytes are considered to arise from several tissues including fetal skin, the fetal urinary tract, umbilical cord, and the inner amniotic surface.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unkown |
| Absence of reprogramming vector(s)? |
Unkown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| HESCA-1 |
Yes |
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Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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