UPSFRi008-A

Cell Line
hPSCreg Name	UPSFRi008-A
Alternative name(s)	PB09
Cell line type	Human induced pluripotent stem cell (hiPSC)
Last update	8th December 2017
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Provider
Generator	Université Paris-Sud 11 (UPSFR) Contact: ESTeam Paris Sud
Derivation country	France
External Databases
Cellosaurus	CVCL_C353
General Information
Publications	Sandt C et al. Identification of spectral modifications occurring during reprogramming of somatic cells. PloS one. 2012;7(4):e30743. Griscelli F et al. Malignant germ cell-like tumors, expressing Ki-1 antigen (CD30), are revealed during in vivo differentiation of partially reprogrammed human-induced pluripotent stem cells. The American journal of pathology. 2012 May;180(5):2084-96. Féraud O et al. Donor Dependent Variations in Hematopoietic Differentiation among Embryonic and Induced Pluripotent Stem Cell Lines. PloS one. 2016;11(3):e0149291.
Is the cell line available in principle?	Yes

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases

We are currently revising this section.

General
Source cell type	amniocyte amniocyte in the OLS A cell of a fetus which is suspended in the amniotic fluid. Amniocytes are considered to arise from several tissues including fetal skin, the fetal urinary tract, umbilical cord, and the inner amniotic surface.
Reprogramming method
Vector type	Integrating
Vector	Virus (Lentivirus)
Genes	POU5F1 SOX2 LIN28 NANOG
Is the used vector excisable?	Unkown
Absence of reprogramming vector(s)?	Unkown
Reprogramming vectors silenced?
Vector free reprogramming
Other
Derived under xeno-free conditions	Unkown
Derived under GMP?	Unkown
Available as clinical grade?	Unkown

Feeder cells

mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040

CO2 Concentration

5 %

Medium

Other medium:

Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Supplements

Marker Expression

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XY
Other Genotyping (Cell Line)

PB09