PB09
UPSFRi008-A
General#
Cell Line |
|
hPSCreg Name | UPSFRi008-A |
Alternative name(s) |
PB09
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
|
Generator |
Université Paris-Sud 11 (UPSFR)
Contact:
ESTeam Paris Sud |
Derivation country | France |
External Databases |
|
Cellosaurus | CVCL_C353 |
General Information |
|
Publications |
|
Is the cell line available in principle? |
Yes |
Donor Information#
General Donor Information |
|
Sex | male |
Phenotype and Disease related information (Donor) |
|
Diseases |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
Source cell type |
amniocyteA cell of a fetus which is suspended in the amniotic fluid. Amniocytes are considered to arise from several tissues including fetal skin, the fetal urinary tract, umbilical cord, and the inner amniotic surface.
|
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Lentivirus) |
Genes | |
Is the used vector excisable? |
Unkown |
Absence of reprogramming vector(s)? |
Unkown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
Unkown |
Derived under GMP? |
Unkown |
Available as clinical grade? |
Unkown |
Culture Conditions#
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
|||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
||||
SSEA-3 |
Yes |
|
||||
SSEA-4 |
Yes |
|
||||
TRA 1-60 |
Yes |
|
||||
HESCA-1 |
Yes |
|
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
Login to share your feedback, experiences or results with the research community.