Cell Line

hPSCreg name SCTi002-A
Cite as:
SCTi002-A (RRID:CVCL_9S50)
Alternative name(s)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 17th May 2024
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Generator STEMCELL Technologies Inc. (SCT)
Derivation country Canada

External Databases

Cellosaurus CVCL_9S50
Wikidata Q54952676

General Information

* Is the cell line readily obtainable for third parties?

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?


Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet.
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information
Alternatives to consent
Alternative consent approval number
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does consent permit access to medical records of the donor? No
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Contact data, institution, or website:
Name of accrediting authority involved? Internal Review - for-profit company
Approval number
Name of accrediting authority involved? Internal Review - for-profit company
Approval number
Do you have obligations to third parties in regard to the use of the cell line? No
Please describe:
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Further constraints on use
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Dr Shinya Yamanaka
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution Not for commercial use, sale or distribution

hIPSC Derivation


Source cell type
A stromal cell that is part_of a bone marrow.
  • bone marrow stromal cell
Source cell origin
the soft tissue that fills the cavities of bones
Source cell type (free text) Bone Marrow Derived Mesenchymal Stromal Cell
Source cell line vendor LONZA
Passage number reprogrammed 14

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming


Selection criteria for clones Clones were picked based on morphology
Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
Passage method Enzyme-free cell dissociation
O2 Concentration 20 %
CO2 Concentration 10 %
Medium mTeSR™ 1


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
TRA 1-81
Differentiation Potency
Definitive Endoderm
Ont Id: EHDAA2_0000337
In vitro directed differentiation
Mesodermal Cell
Ont Id: CL_0000222
In vitro directed differentiation
Neural Stem Cell
Ont Id: CL_0000047
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)