STiPS-M001

General#

Cell Line

hPSCreg Name SCTi002-A
Alternative name(s)
STiPS-M001
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator STEMCELL Technologies Inc. (SCT)
Derivation country Canada

External Databases

Cellosaurus CVCL_9S50

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
stromal cell of bone marrow
a stromal cell that is part_of a bone marrow
Synonyms
  • bone marrow stromal cell
Source cell origin
bone marrow
the soft tissue that fills the cavities of bones
Synonyms
  • medulla ossium
  • medulla ossea
Source cell type (free text) Bone Marrow Derived Mesenchymal Stromal Cell
Source cell line vendor LONZA
Passage number reprogrammed 14

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Selection criteria for clones Clones were picked based on morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
O2 Concentration 20 %
CO2 Concentration 10 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Unkown
NANOG
Unkown
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Unkown
TRA 1-60
Unkown
TRA 1-81
Yes
ZFP42 (REX-1)
Unkown
Differentiation Potency
Definitive Endoderm
Ont Id: EHDAA2_0000337
In vitro directed differentiation
Mesodermal Cell
Ont Id: CL_0000222
In vitro directed differentiation
Neuronal Stem Cell
Ont Id: CL_0000047
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)