Generation of a human induced pluripotent stem cell line (SIAISi011-A) from a 61-year-old Chinese Han healthy female donor
Peripheral blood mononuclear cells (PBMC) were donated by a healthy 61-year old Chinese Han female. Human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors were used to reprogram her PBMCs with the non-integrating episomal vector system. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs and their ability to differentiate spontaneously three germ layers in vitro. The iPSC line displayed a normal karyotype. Our model offers the possibility to be used a control in pathological mechanism studies. Copyright © 2021. Published by Elsevier B.V.
|Authors||Zhang W, Hua Y, Di W, Guo J, Dai Q, Zhao J, Zhang B, Wang Y|
|Journal||Stem cell research|
|Publication Date||2021 Mar;51:102173|