NYSCFe002-A

NY0002-01-ES-001

General#

Cell Line

hPSCreg Name NYSCFe002-A
Alternative name(s)
NY0002-01-ES-001
Cell line type Human embryonic stem cell (hESC)
Last update 14th November 2017

Provider

Generator New York Stem Cell Foundation Research Institute (NYSCF)
Owner New York Stem Cell Foundation Research Institute (NYSCF)
Derivation country United States

External Databases

BioSamples SAMEA104618140

General Information

Is the cell line available in principle? Available with restrictions: Available for commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104618141

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hESC Derivation#

Date of derivation 2016-04-11
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique 0
Cell isolation Laser
Cell seeding Isolated ICM
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DEMEM-F12/ Glutamax (1X)
Main protein source: Albumine
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
TRA 1-81
Yes
TRA 1-60
Yes
SSEA-4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Smooth Muscle Actin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUJ-1
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 8

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)