Generation of human induced pluripotent stem cell lines from two down syndrome patients, including a down syndrome/Alzheimer's disease case (FLENIi002-A) and a beta-amyloid-resistant case (FLENIi003-A)
Summary
Two human induced pluripotent stem cell (hiPSC) lines, FLENIi002-A and FLENIi003-A, were generated from peripheral blood mononuclear cells (PBMCs) using the lentiviral-hSTEMCCA-loxP vector. FLENIi002-A was derived from a 52-year-old Down syndrome patient with Alzheimer's disease and amyloid-beta brain accumulation. FLENIi003-A was derived from a cognitively unimpaired 51-year-old Down syndrome patient exhibiting no brain amyloid-beta deposition. Both lines retained the trisomy 21 genotype, and their pluripotency and differentiation potential were confirmed. Copyright © 2025 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Clas GS, Marazita M, Pertierra L, Vazquez S, Isaja L, Rodríguez-Varela S, Mucci S, Helou B, Tapajóz F, Magrath N, Bérgamo Y, Allegri R, Sevlever GE, Scassa ME, Surace EI, Romorini L |
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| Journal | Stem cell research |
| Publication Date | 2025 May 1;86:103728 |
| PubMed | 40334312 |
| DOI | 10.1016/j.scr.2025.103728 |