01279

General#

Cell Line

hPSCreg Name CDIi001-A
Alternative name(s)
01279
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Cellular Dynamics International, a FUJIFILM Company (CDI)
Derivation country United States

External Databases

BioSamples SAMEA104012950
Cellosaurus CVCL_IT57

General Information

Publications
Projects
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104012951

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
Age of donor (at collection) 55-59

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones Clones were identified by proprietary assay to determine pluripotency.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Feeder cells
No
Medium mTeSR™ 2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
LIN28
Yes
Undifferentiated cells were analyzed by proprietary assays approved by California Institute of Regenerative Medicine (CIRM) as described in the attached publication. Pluripotency of established iPSC cultures were tested by qPCR on bulk samples using 48 genes including standard pluripotency genes (listed above). Samples were assigned a passing score after analysis with a non-supervised classifier based on an appropriate sample set.
Method documentation
Methods for hPSCreg.pdf
Methods for hIPSC characterization
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro directed differentiation
Marker Expressed
Alpha anti trypsin
Yes
Nestin
No
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
Cardiac Troponin T
Yes
Nestin
No
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
beta III Tubulin
Yes
Nestin
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)