The cell line is not validated yet.

General#

Cell Line

hPSCreg Name
SCHi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 26th October 2021
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Provider

Generator Severance Children’s Hospital (SCH)

External Databases

BioSamples SAMEA10405028
CLO CLO_0103534

General Information

* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Adrenoleukodystrophy
Synonyms
  • Adrenoleukodystrophy
  • Schilder Disease

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA10405029

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: jihun264@yuhs.ac
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Yonsei University Health System, Severance Hospital, Institutional Review Board
Approval number 4-2016-0194
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Induced Pluripotent Stem Cell
Adult cells that have been genetically reprogrammed to an embryonic stem cell-like state by the expression of genes and factors important for maintaining the defining properties of embryonic stem cells.
Synonyms
  • iPSC
  • Induced Pluripotent Stem Cell
Source cell origin
Skin
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
  • Skin
  • Human Skin
  • SKIN
  • Integument
  • Skin, total
  • Skin, NOS
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Sendai Virus)
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotype

Other Genotyping (Cell Line)