HAD 4
HADe004-A
General#
Cell Line |
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| hPSCreg Name | HADe004-A |
| Alternative name(s) |
HAD 4
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 20th January 2020 |
| User feedback | |
Provider |
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| Generator | Hadassah University Hospital (HAD) |
| Owner | Hadassah Human Embryonic Stem Cell Research Center |
| Distributors | |
| Derivation country | Israel |
External Databases |
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| Cellosaurus | CVCL_C075 |
| BioSamples | SAMEA6613187 |
| CLO | CLO_0102575 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
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Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA6613348 |
Ethics#
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Hadassah IRB Committee |
| Approval number | 16-23.04.04 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
hESC Derivation#
| Date of derivation | 2006-02-24 |
| Supernumerary embryos from IVF treatment? |
No
Other hESC source:
IVF
|
| PGD Embryo? |
Yes |
| Derived under xeno-free conditions? |
No |
| Derivation under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Feeder cells |
foreskin |
| O2 Concentration | 4 % |
| CO2 Concentration | 5 % |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| TUBB3 |
Yes |
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| SOX17 |
Yes |
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| desmin |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
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Other Genotyping (Cell Line) |
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