General

Cell Line
hPSCreg name	HADe004-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	HADe004-A (RRID:CVCL_C075)
Alternative name(s)	HAD 4
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	14th April 2022
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Provider
Generator	Hadassah University Hospital (HAD) Contact: Hadassah University Medical Center - Dept. of Obstetrics & Gynecology
Owner	Hadassah Human Embryonic Stem Cell Research Center
Distributors	Hadassah Human Embryonic Stem Cell Research Center Hadassah University Hospital (HAD)
Derivation country	Israel
External Databases
Cellosaurus	CVCL_C075
BioSamples	SAMEA6613187
Wikidata	Q54872333
General Information
Publications	Turetsky T et al. Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis. Human reproduction (Oxford, England). 2008 Jan;23(1):46-53. Lefler S et al. Familial Dysautonomia (FD) Human Embryonic Stem Cell Derived PNS Neurons Reveal that Synaptic Vesicular and Neuronal Transport Genes Are Directly or Indirectly Affected by IKBKAP Downregulation. PloS one. 2015;10(10):e0138807.
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Familial Dysautonomia Synonyms Riley-Day Syndrome HSAN 3 Neuropathy, Hereditary Sensory and Autonomic, Type III Hereditary Sensory and Autonomic Neuropathy Type III Familial Dysautonomia HSAN III Riley- Day Familial dysautonomia hereditary sensory and autonomic neuropathy type III show more synonyms show less synonyms Genetic variants ELP1 (target) Is the variant homozygous or heterozygous? Homozygous Free text Sequence variation: Homozygous for ELP1 c.2204+6T>C (2507+6T>C) (IVS20+6T>C) (Ex20del); splice donor mutation (PubMed=26437462)
External Databases (Donor)
BioSamples	SAMEA6613348

Ethics

Was the embryo established purely for research purposes?	No
Have both parents consented to the use of the embryo for ESC derivation?	Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
How may genetic information associated with the cell line be accessed?	Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Hadassah IRB Committee
Approval number	16-23.04.04
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells?	No

hESC Derivation

Date of derivation	2006-02-24
Supernumerary embryos from IVF treatment?	No Other hESC source: IVF
PGD Embryo?	Yes
Derived under xeno-free conditions?	No
Derivation under GMP?	No
Available as clinical grade?	No

Culture Conditions

Feeder cells	foreskin
O2 Concentration	4 %
CO2 Concentration	5 %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
TUBB3	Yes
SOX17	Yes
desmin	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vivo teratoma

In vitro directed differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vivo teratoma

In vitro directed differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vivo teratoma

In vitro directed differentiation

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY
Other Genotyping (Cell Line)