Generation and characterization of a human iPSC line expressing EGFP-tagged CDH1, KSCBi002-A-3
Summary
E-cadherin, a transmembrane protein, is essential for maintaining the integrity and structure of human pluripotent stem cells (hPSCs) by facilitating strong cell-cell adhesion and communication, which is crucial for their colony formation and pluripotency. Here, we used the CRISPR/Cas9 system to introduce the enhanced green fluorescent protein (EGFP)-tagged CDH1 into the AAVS1 locus, a safe harbour site, of human induced pluripotent stem cells (hiPSCs). The engineered cell line, KSCBi002-A-3, expressed functional CDH1-EGFP fusion protein, exhibited normal cell morphology, maintained a normal karyotype, and retained pluripotent state. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Oh JY, Yoo DH, Im YS, Kim YO |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Oct;80:103510 |
| PubMed | 39121651 |
| DOI | 10.1016/j.scr.2024.103510 |