CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout
Summary
The receptor tyrosine kinase c-KIT (CD117) has a key role in hematopoiesis and is a marker for endothelial and cardiac progenitor cells. In vivo, deficiency of c-KIT is lethal and therefore using CRISPR/Cas9 editing we generated heterozygous and homozygous c-KIT knockout human embryonic stem cell (ES cell) lines. The c-KIT knockout left ES cell pluripotency unaffected as shown by immunofluorescence and trilineage differentiation potential. Heterozygous and homozygous c-KIT knockouts showed complete loss of exon 17, resulting in ablation of c-KIT protein from the cell surface. c-KIT knockout ES cells provide a valuable tool for further investigating c-KIT biology. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | de Toledo MAS, Fu X, Kluge F, Götz K, Schmitz S, Wanek P, Schüler HM, Pannen K, Chatain N, Koschmieder S, Brümmendorf TH, Zenke M |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Apr;60:102732 |
| PubMed | 35279545 |
| DOI | 10.1016/j.scr.2022.102732 |