CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout


The receptor tyrosine kinase c-KIT (CD117) has a key role in hematopoiesis and is a marker for endothelial and cardiac progenitor cells. In vivo, deficiency of c-KIT is lethal and therefore using CRISPR/Cas9 editing we generated heterozygous and homozygous c-KIT knockout human embryonic stem cell (ES cell) lines. The c-KIT knockout left ES cell pluripotency unaffected as shown by immunofluorescence and trilineage differentiation potential. Heterozygous and homozygous c-KIT knockouts showed complete loss of exon 17, resulting in ablation of c-KIT protein from the cell surface. c-KIT knockout ES cells provide a valuable tool for further investigating c-KIT biology. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors de Toledo MAS, Fu X, Kluge F, Götz K, Schmitz S, Wanek P, Schüler HM, Pannen K, Chatain N, Koschmieder S, Brümmendorf TH, Zenke M
Journal Stem cell research
Publication Date 2022 Apr;60:102732
PubMed 35279545
DOI 10.1016/j.scr.2022.102732

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