mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions
Summary
Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA). © 2020. Springer Science+Business Media New York.
| Authors | Jeriha J, Kolundzic N, Khurana P, Perez-Dominguez A, Ilic D |
|---|---|
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Publication Date | 2022;2454:665-674 |
| PubMed | 32567019 |
| DOI | 10.1007/7651_2020_302 |