HuES15
HVRDe015-A
General
Donor Information
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases |
Ethics
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | NIHhESC-09-0028 |
How may genetic information associated with the cell line be accessed? |
hESC Derivation
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
No |
Expansion status |
3 |
ICM morphology |
C |
Trophectoderm morphology |
g |
ZP removal technique | Chemical |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Culture Conditions
Passage method | Enzymatically | ||||||
CO2 Concentration | 5 % | ||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 10 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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DNMT3B |
Yes |
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GABRB3 |
Yes |
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GDF3 |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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TDGF1 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 12
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Other Genotyping (Cell Line) |
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