ACT-14

General#

Cell Line

hPSCreg Name ACTe003-A
Alternative name(s)
ACT-14
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Advanced Cell Technology, Inc. - Cellular Reprogramming unit (ACT)
Derivation country United States

External Databases

Cellosaurus CVCL_B523

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes

Culture Conditions#

Passage method Enzymatic, Mechanical
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source:
Supplements
glutamax
bFGF
LiF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)