HES-4
ESIBIe004-A
General
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
||||||
| Passage method | Enzymatically and Mechanically | ||||||
| O2 Concentration | 21 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| GCTM2 |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
|
|||
| TRA 1-60 |
Yes |
|
|
|||
| DNMT3B |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| ZFP42 (REX-1) |
No |
|
||||
| SOX2 |
Yes |
|
||||
| TDGF1 |
Yes |
|
||||
| Alkaline Phosphatase |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| TRA 1-81 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 99
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.