CEBe002-A-1

AS034.1

General

Cell Line

hPSCreg name CEBe002-A-1
Cite as:
CEBe002-A-1 (RRID:CVCL_B341)
Alternative name(s)
AS034.1
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Takara Bio Europe AB (former Cellartis) (CEB)
Derivation country Sweden

External Databases

BioSamples SAMEA104620496
Cellosaurus CVCL_B341
Wikidata Q54750602

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA104620497

Ethics

Also have a look at the ethics information for the parental line CEBe002-A .
Alternatives to consent

hESC Derivation

The source cell information can be found in the parental cell line CEBe002-A.

Culture Conditions

Feeder cells ES-C57BL/6
Cellfinder Ont Id: CLO_0002948
O2 Concentration 20 %
CO2 Concentration 5 %
Medium VitroHES
Supplements
Serum Replacement
hrbFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification