CEBe002-A-1

AS034.1

General#

Cell Line

hPSCreg Name
CEBe002-A-1
Alternative name(s)
AS034.1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Takara Bio Europe AB (former Cellartis) (CEB)
Derivation country Sweden

External Databases

BioSamples SAMEA104620496
Cellosaurus CVCL_B341
CLO CLO_0102901
Wikidata Q54750602

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA104620497

Ethics#

Also have a look at the ethics information for the parental line CEBe002-A .
Alternatives to consent

hESC Derivation#

The source cell information can be found in the parental cell line CEBe002-A.

Culture Conditions#

Feeder cells ES-C57BL/6
Cellfinder Ont Id: CLO_0002948
O2 Concentration 20 %
CO2 Concentration 5 %
Medium VitroHES
Supplements
Serum Replacement
hrbFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
UBERON_0000924
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#