AS034.1	
    			    			
                CEBe002-A-1            
            
        General
Donor Information
General Donor Information | 
					|
| Sex | male | 
Phenotype and Disease related information (Donor) | 
					|
| Diseases | |
External Databases (Donor) | 
						|
| BioSamples | SAMEA104620497 | 
Ethics
Also have a look at the ethics information for the parental line
        				
		
			CEBe002-A		
        				.
        			        
        			        			        			        			| Alternatives to consent | 
hESC Derivation
| 
							 The source cell information can be found in the parental cell line
							CEBe002-A. 
						 | 
					
Culture Conditions
| Feeder cells | 
																								ES-C57BL/6 Cellfinder Ont Id: CLO_0002948  | 
						|||||||||
| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium | 
								VitroHES		
				 Supplements 
				
  | 
						
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles | 
| POU5F1 (OCT-4) | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                
							                 | 
						                |||
| SSEA-3 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                ||||
| SSEA-4 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                ||||
| TRA 1-60 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                ||||
| TRA 1-81 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                ||||
| SSEA-1 | 
											 No 						                 | 
																                
						                
						                	 | 
						                ||||
| Alkaline Phosphatase | 
											 Yes 						                 | 
																                
						                
							                 | 
						                
Differentiation Potency
Microbiology / Virus Screening | 
						|
| HIV 1 | Negative | 
| Hepatitis B | Negative | 
| Hepatitis C | Negative | 
Genotyping
Karyotyping (Cell Line) | 
					|
Other Genotyping (Cell Line) | 
					

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