ERAMUC-2

General#

Cell Line

hPSCreg Name ULBe005-A
Alternative name(s)
ERAMUC-2
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Université Libre de Bruxelles (ULB)
Derivation country Belgium

External Databases

Cellosaurus CVCL_9009

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases
Disease associated phenotypes
  • Monogenetic disease

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2006-03-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
ZP removal technique Chemical
Cell isolation Mechanical
Cell seeding Embryo

Culture Conditions#

Passage method Mechanically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: %
Supplements
glutamax
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
hTERT
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)