Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system


The human specific gene ARHGAP11B is preferentially expressed in neural progenitors of fetal neocortex and plays a key role in the evolutionary expansion of the neocortex. Here, we generated a homozygous ARHGAP11B knockout human induced pluripotent stem cell (hiPSC) line through CRISPR/Cas9 gene editing system. ARHGAP11B deficient cell line maintained a normal karyotype (46, XX), expressed pluripotency markers, and showed the capability to spontaneously differentiate into all three germ layers in vivo. The ARHGAP11B knockout cell line can provide a new cell model for studying the evolution of human neocortex. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Liu Y, Jin Y, Chen T, Wu Y, Peng X, Li W, Wei S, Chen M, Zou Q, Guo S, Xu J, Tang C, Zhou X
Journal Stem cell research
Publication Date 2022 May;61:102764
PubMed 35358830
DOI 10.1016/j.scr.2022.102764

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