Generation of a homozygous ARHGAP11B knockout hiPSC line by CRISPR/Cas9 system
Summary
The human specific gene ARHGAP11B is preferentially expressed in neural progenitors of fetal neocortex and plays a key role in the evolutionary expansion of the neocortex. Here, we generated a homozygous ARHGAP11B knockout human induced pluripotent stem cell (hiPSC) line through CRISPR/Cas9 gene editing system. ARHGAP11B deficient cell line maintained a normal karyotype (46, XX), expressed pluripotency markers, and showed the capability to spontaneously differentiate into all three germ layers in vivo. The ARHGAP11B knockout cell line can provide a new cell model for studying the evolution of human neocortex. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Liu Y, Jin Y, Chen T, Wu Y, Peng X, Li W, Wei S, Chen M, Zou Q, Guo S, Xu J, Tang C, Zhou X |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 May;61:102764 |
| PubMed | 35358830 |
| DOI | 10.1016/j.scr.2022.102764 |