Direct whole-cell patch-clamp recordings from small boutons in rodent primary neocortical neuron cultures


Direct electrical recordings from conventional boutons in the mammalian central nervous system have proven challenging due to their small size. Here, we provide a protocol for direct whole-cell patch-clamp recordings from small presynaptic boutons of primary dissociated cultured neurons of the rodent neocortex. We describe steps to prepare primary neocortical cultures and recording pipettes, followed by identifying boutons and establishing a whole-cell bouton recording. We then provide details on precise pipette capacitance compensation required for high-resolution current-clamp recordings from boutons. For further details on the use and execution of this protocol, please refer to Ritzau-Jost et al.1. Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Authors Ritzau-Jost A, Nerlich J, Kaas T, Krueger M, Tsintsadze T, Eilers J, Barbour B, Smith SM, Hallermann S
Journal STAR protocols
Publication Date 2023 Mar 14;4(2):102168
PubMed 36920913
PubMed Central PMC10026040
DOI 10.1016/j.xpro.2023.102168

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