|Cell line type
|Human induced pluripotent stem cell (hiPSC)
|19th August 2022
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|Center for Regenerative Therapies Dresden (CRTD)
|Department of Pediatrics, Medical Faculty, TU Dresden
|* Is the cell line readily obtainable for third parties?
General Donor Information
Phenotype and Disease related information (Donor)
|A disease was diagnosed.
|Other cell lines of this donor
External Databases (Donor)
|Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?
|Was the consent voluntarily given?
|Has the donor been informed that participation will not directly influence their personal treatment?
|Can you provide us with a copy of the Donor Information Sheet provided to the donor?
|Do you (Depositor/Provider) hold the original Donor Consent Form?
|Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.
|Does consent explicitly allow the derivation of pluripotent stem cells?
|Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?
|How may genetic information associated with the cell line be accessed?
|Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?
|Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?
|Name of accrediting authority involved?
|Ethics Committee of the Medical Faculty Carl Gustav Carus, TUD
|EK169052010 and EK386102017
|For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
|Source cell type
|Is reprogramming vector detectable?
Vector free reprogramming
|Selection criteria for clones
|Derived under xeno-free conditions
|Derived under GMP?
|Available as clinical grade?
Base medium: DMEM F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
|Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
|Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
|Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Analysis of Undifferentiated Cells
Microbiology / Virus Screening
Karyotyping (Cell Line)
|Has the cell line karyotype been analysed?
Karyotyping method: G-Banding
Other Genotyping (Cell Line)