General

Cell Line

hPSCreg Name
CRTDi006-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th August 2022
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Provider

Generator Center for Regenerative Therapies Dresden (CRTD)
Owner Department of Pediatrics, Medical Faculty, TU Dresden

External Databases

BioSamples SAMEA12596512
CLO CLO_0103944
Cellosaurus CVCL_C1P2
Wikidata Q114311269

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Aicardi-Goutieres Syndrome 1
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Encephalopathy, Familial Infantile, with Intracranial Calcification and Chronic Cerebrospinal Fluid Lymphocytosis
  • Cree Encephalitis
  • AGS1
  • Pseudotoxoplasmosis Syndrome
  • Aicardi-Goutieres Syndrome 1
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA12595589

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of the Medical Faculty Carl Gustav Carus, TUD
Approval number EK169052010 and EK386102017
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Fibroblasts

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones mophology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Feeder cells Fibroblasts
Passage method Enzymatically
Collagenase
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
SSEA-4
Yes
Morphology pictures
scalebar 500 µm
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2 (SMA)
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3 (TUJ1)
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
iPSC
Passage number: 87
Karyotyping method: G-Banding

Other Genotyping (Cell Line)