Generation of human-induced pluripotent stem cell line from PBMC of healthy donor using integration-free Sendai virus technology

Summary

We developed a well-characterized human induced pluripotent stem cell (iPSC) line obtained from healthy individuals' peripheral blood mononuclear cells (PBMC). The PBMCs were primed and reprogrammed using a non-integrating sendai viral vector, and the iPSC lines demonstrated complete differentiation capacity. This line, YBLi004-A, is available and registered in the human pluripotent stem cell registry. The line's legitimacy was validated using pluripotent marker expression, in vitro differentiation into three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis. This iPSC line could be used as a healthy control for studies involving disease-specific-iPSCs, e.g. drug toxicity and efficacy testing. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Sarkar J, Dhepe S, Shivalkar A, Kuhikar R, More S, Konala VBR, Bhanushali P, Khanna A
Journal Stem cell research
Publication Date 2024 Jun;77:103402
PubMed 38547666
DOI 10.1016/j.scr.2024.103402

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