CyT49

General#

Cell Line

hPSCreg Name VCYTe001-A
Alternative name(s)
CyT49
Cell line type Human embryonic stem cell (hESC)
Last update 10th April 2017
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Provider

Generator ViaCyte, Inc. (VCYT)
Owner Stem Cell Derivation
Distributors
Derivation country United States

External Databases

BioSamples SAMEA17469418
Cellosaurus CVCL_B850
NIHhESC NIHhESC-10-0041

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history No
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XY

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA17470168

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2005-11-02
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 2
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Chemical
Cell isolation None
Cell seeding Embryo
Derived under xeno-free conditions?
No
Derivation under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Non coated
Feeder cells Human Dermal Fibroblasts
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 8 %
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Alanyl-L-Glutamine 1 %
Non-Essential Amino Acids 1 %
Penicillin/Streptomycin 1 %
2-Mercaptoethanol 0.1 mM
Activin A 25 ng/ml
FGF2 10 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)