Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9


The role of Neurochondrin (NCDN) in humans is not well understood. Mice with a conditional Ncdn knock-out show epileptic seizures, depressive-like behaviours and impaired spatial learning. Using CRISPR/Cas9, we generated a Neurochondrin deficient human iPSC line KICRi002-A-3 carrying a homozygous 752 bp deletion / 2 bp insertion in the NCDN gene. The iPSC line maintained a normal 46,XY karyotype, expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. Off-target editing was excluded and Neurochondrin expression was not detectable. The iPSC line offers a valuable resource to study the role of Neurochondrin during human neurogenesis. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Fatima A, Schuster J, Akram T, Sobol M, Hoeber J, Dahl N
Journal Stem cell research
Publication Date 2020 Apr;44:101758
PubMed 32203915
DOI 10.1016/j.scr.2020.101758

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