Induced pluripotent stem cell line heterozygous for p.R501X mutation in filaggrin: KCLi003-A


We have generated an induced pluripotent stem cell (iPSC) line KCLi003-A (iOP101) from epidermal keratinocytes of a female donor, heterozygous for the loss-of-function mutation p.R501X in the filaggrin gene (FLG), using non-integrating Sendai virus vectors. Derivation and expansion of iPSCs were performed under xeno-free culture conditions. Characterization and validation of KCLi003-A line included molecular karyotyping, mutation screening using restriction enzyme digestion, next generation sequencing (NGS), while pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and by in vivo teratoma assay. Copyright © 2019 King's College London. Published by Elsevier B.V. All rights reserved.

Authors Kolundzic N, Khurana P, Devito L, Jeriha J, Marx J, Hobbs C, Wong XFCC, Common JEA, Liovic M, Dubrac S, Gruber R, Schmuth M, Mauro TM, Ilic D
Journal Stem cell research
Publication Date 2019 Aug;39:101527
PubMed 31408836
DOI 10.1016/j.scr.2019.101527

Research Projects

Cell Lines