DKH005i-A, DKHi005-A

General

Cell Line

hPSCreg name KSCBi010-A
Cite as:
KSCBi010-A (RRID:CVCL_WR22)
Alternative name(s)
DKH005i-A, DKHi005-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
KMUGMCi003-A
(KMUGMCi003ERCC2, KN610)
Donor diseases:
Trichothiodystrophy 1, Photosensitive
KMUGMCi005-A
(KMUGMCi005TMC8, KN627)
Donor diseases:
Epidermodysplasia Verruciformis
INSRMi021-A
(PC177 3c14)
Donor diseases:
myofibrillar myopathy 1
PFIZi032-A
(B214c8)
Donor's gene variants:
TSC2
Donor diseases:
Tuberous Sclerosis
AIBNi016-A
(SPG2-S376C1)
Donor diseases:
hereditary spastic paraplegia 56
IMAGINi022-A
(IMAGINE022)
Donor diseases:
Waardenburg syndrome
ITXi013-A
(PT-R406W)
Donor's gene variants:
DES
Donor diseases:
Desminopathy
ITXi013-B
(IC-R406)
Donor's gene variants:
DES
Donor diseases:
Desminopathy
CMCi001-A
(CMC-KIN-hiPSC)
Donor diseases:
karyomegalic interstitial nephritis
PCIi026-A
(PC085)
Donor diseases:
Waardenburg syndrome
IMBAi017-A
(B002-ARID1B#8)
Donor diseases:
Coffin-Siris Syndrome
UOMi010-A
(ANCS-hiPSC-HPP5)
Donor diseases:
Hypophosphatasia
UOMi011-A
(ANCS-hiPSC-HPP6)
Donor diseases:
Hypophosphatasia
PFIZi023-A
(B217c8)
Donor's gene variants:
GPR56, GPR56
Donor diseases:
Bilateral Frontoparietal Polymicrogyria
PCIi025-A
(PC084)
Donor diseases:
Waardenburg syndrome
PCIi032-A
(PC087)
Donor diseases:
Waardenburg Syndrome
BCHNi001-A
(BCHi001-A)
Donor diseases:
Bardet-Biedl Syndrome
MUBi002-A
(CF 001)
Donor diseases:
Cystic fibrosis
SZGJMSi001-A
(ZLP-2)
Donor diseases:
Schizophrenia
SCVIi091-A
(SCVI2617)
Donor diseases:
transthyretin amyloidosis
KMUGMCi002-A
(KMUGMCi002NIPBL)
Donor diseases:
Cornelia De Lange Syndrome
KMUGMCi004-A
(KMUGMCi004APC, KN615)
Donor diseases:
familial adenomatous polyposis
MRIi026-A
(Noonan 3d)
Donor diseases:
Noonan Syndrome
ZJSHi001-A
(ZJSHi-KCNB1)
Donor's gene variants:
KCNB1
Donor diseases:
developmental and epileptic encephalopathy, 26
WCHi006-A
Donor diseases:
Schizophrenia
CABi003-A
(DH05, AMDdh05-MiPS4F16)
Donor diseases:
Age-Related Macular Degeneration
USPi006-A
(DFNA58-ipsc-dup1-cl2)
Donor diseases:
autosomal dominant nonsyndromic deafness 58
USPi007-A
(DFNA58-ipsc-dup2-cl5)
Donor diseases:
autosomal dominant nonsyndromic deafness 58
SCTCi011-A
(IPS18-00072)
Donor diseases:
age-related macular degeneration
UKKi034-A
(NP0079-A, NP0079-7B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
long QT syndrome 1
WCHi003-A
Donor diseases:
Schizophrenia
UKKi034-B
(NP0079-B, NP0079-15B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
long QT syndrome 1
UKKi034-C
(NP0079-C, NP0079-16H)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
long QT syndrome 1
CMCi009-A
(CMC-BHD-001)
Donor's gene variants:
FLCN
Donor diseases:
Birt-Hogg-Dube Syndrome
SCTCi015-A
(IPS19-00051)
Donor diseases:
Age-Related Macular Degeneration
Last update 9th January 2024
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator National Stem Cell Bank (KSCB)
Owner National Stem Cell Bank (KSCB)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA5892403
Cellosaurus CVCL_WR22
Wikidata Q95980264

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Loken Senior syndrome
  • renal-retinal syndrome

External Databases (Donor)

BioSamples SAMEA5892404

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Yonsei university college of medicine, seoul, south korea
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Yonsei university college of medicine, seoul, south korea
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Yonsei university health system, severance hospital, institutional review board
Approval number 4-2016-1158
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Yonsei university health system, severance hospital, institutional review board
Approval number 4-2016-1158
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
SOX2
Yes
ZFP42 (REX-1)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)